Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 May 14;204(5):971–977. doi: 10.1084/jem.20052078

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Targeted disruption of NF90 impairs T cell IL-2 gene expression and T cell homeostasis. (A) Thymocytes and splenocytes were isolated from adult NF90^+/+ and NF90^+/− littermate mice and stimulated with for 6–18 h with PMA/Iono, and secreted IL-2 was measured by ELISA. The data are representative of two independent pairs of littermates. NF90^+/− IL-2 reduction was significant (P < 0.05). (B) Thymocytes and splenocytes were isolated from two newborn NF90^+/+, two NF90^+/−, and one NF90^−/− littermate mice and stimulated for 6 h with PMA/Iono, and IL-2 was measured. IL-2 reduction was significant (P < 0.001). Nonstimulated cells produced no detectable IL-2. (C) Immune reconstitution analysis of NF90-RAGγ mice. Representative flow cytometry of CD4⁺ and CD8⁺ lymphocytes in the thymus and spleen of NF90^−/−- and NF90^+/+-RAG^−/−/IL-2Rγ^−/− chimeric mice. NF90^−/−-RAGγ chimera yielded 10⁷ thymocytes, 4.2 × 10⁸ splenocytes, and 1,666 peripheral lymphocytes per mm³, and NF90^+/+-RAGγ chimera yielded 10⁷ thymocytes, 4.5 × 10⁸ splenocytes, and 3,455 peripheral lymphocytes per mm³. Recipient mice received 900 rads conditioning radiation and were killed at 14 wk after transplantation with NF90 E14 fetal liver cells. Peripheral blood lymphocyte subset analyses of the same mice at 9 wk after transplantation demonstrated a nearly complete absence of circulating CD4⁺ and CD8⁺ T cells associated with the NF90^−/− genotype (row 3). Spleen cells were gated on CD3 and analyzed for expression of CD4 and CD25 in nonstimulated conditions (row 4) and after 24 h of stimulation with anti-CD3 (row 5). The percentage of cells in each quadrant is shown. (D) Impaired IL-2 secretion in NF90^−/−-RAGγ compared with NF90^+/−- or NF90^+/+-RAGγ chimeric mice. Single-cell suspensions of thymocytes, splenocytes, and CD4⁺ T cells from individually numbered chimeras were stimulated with PMA/Iono or anti-CD3 + CD28. After 16 h, secreted IL-2 was measured. IL-2 reduction in the NF90^−/−-RAGγ chimeric T cells was significant (P < 0.02). Data in A, B, and D represent the mean ± SD.