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. 2007 May 14;204(5):1227–1235. doi: 10.1084/jem.20070145

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — LIMP-2 regulates ID integrity by regulating the binding of phosphorylated β-catenin to cadherin. Immunoblot (IB) of lysates of neonatal rat cardiac myocytes that were treated either with shLIMP-2 or control shRNA (B). After 10 d of culture, cardiac myocytes show a 92% knockdown of LIMP-2 protein. Equal protein loading was confirmed by GAPDH. Representative immunoblots (IB) show diminished levels of P-β-catenin after IP with anti–pan-cadherin in LIMP-2 KO tissue lysates (A) as well as in shLIMP-2 cell lysates (C) as compared with control. P-β-catenin in total shLIMP-2 and control protein lysates was comparable (C). Quantification of immunoblots (n = 3 per group for control and LIMP-2 KO tissue lysates; n = 4 per group for control and shLIMP-2 cell lysates) is shown in D. Overexpression of LIMP-2 in neonatal rat cardiac myocytes increases levels of P-β-catenin bound to pan-cadherin (E and F). *, P < 0.05 versus control; **, P < 0.001 versus control.