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. 2007 May 14;204(5):1057–1069. doi: 10.1084/jem.20070075

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Copyright © 2007, The Rockefeller University Press

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Figure 2. — Phenotype of Ly-6C⁺ and Ly-6C⁻ MOs/MPs during muscle regeneration. Ly-6C⁺ and Ly-6C⁻ MO/MP subsets were isolated by cell sorting at various times after injury. (A) Representative example of cell sorting of Ly-6C⁻ (middle) and Ly-6C⁺ (right) cells from whole MOs/MPs (left) at day 4 after injury. (B) Ki67 immunostaining is expressed as the percentage of isolated MOs/MPs. (C) Expression of TNF-α, IL-1β, TGF-β1, and IL-10 was analyzed by RT-PCR in isolated populations at day 4 after injury. Corresponding band intensities are given as the means of three experiments.