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. 2007 May 14;204(5):1057–1069. doi: 10.1084/jem.20070075

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — Effects of i.m. DT injection on skeletal muscle regeneration. Notexin (Nx) was injected into the TA of CD11b-DTR mice at day 0, and DT was injected in the same muscle at day 5 (A) and days 5 and 6 (B and C). (A) 24 h after a single i.m. DT injection, CD45⁺ cells were isolated from muscle and analyzed for F4/80 expression. Red circles enclose FSC^hi/F4/80^hi cells. (B) Reconstituted whole view of TA muscle at day 10 after Notexin injection (hematoxylin and eosin staining), presenting hallmarks of secondary regeneration restricted to the site of needle puncture (arrow). (right) Hatched area represents the area excluded from analysis. Bar, 200 μm. (C) PBS- and DT-injected muscles were analyzed at day 10 after injury for myofiber diameter evaluation (hematoxylin and eosin staining). Quantified results are given for three independent experiments, with each point corresponding to a field (red bars represent the means). Bar, 50 μm.