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. 2007 May 14;204(5):1207–1216. doi: 10.1084/jem.20070087

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Normal irradiation-induced foci formation in patients' fibroblasts. Primary fibroblasts from P4 either untreated or irradiated with 2 Gy were labeled with anti-MRE11, anti-53BP1, or anti-γH2AX mouse monoclonal antibodies and anti-MDC1 rabbit polyclonal antibody followed by anti-mouse Alexa Fluor 488, anti-mouse Alexa Fluor 546,or anti-rabbit Alexa Fluor 488. Nuclei were stained with DAPI. The same results were obtained with fibroblasts from patients P2, P3, and P4 and EBV B cell lines from patients P1, P2, and P5. Similar foci formation was observed at 5 Gy of irradiation.