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. 1998 Jul 21;95(15):8969–8974. doi: 10.1073/pnas.95.15.8969

Figure 3.

Figure 3

Single-channel recordings in outside-out patches from HEK293 cells transfected with NR1–1a/NR2A show that (RS)-DHPG acts as a coagonist with NMDA. (A) Single-channel openings induced by application of 5 μM NMDA and 5 μM glycine to an outside-out patch held at a membrane potential of −70 mV. Typically 500–1,500 channel openings were measured for each recording and the amplitude and open period histograms constructed and fitted with the sum of two Gaussian components or two exponential components, respectively. For this recording the main conductance level had a single-channel conductance of 46 pS and a mean open time of 3.3 ms (B) Single-channel openings recorded in the same patch as illustrated in A., in the presence of 5 μM NMDA and 1 mM DHPG. The main conductance level had a single-channel conductance of 44 pS and a mean open time of 2.9 ms. Calibration bars = 100 ms (x axis) and 3 pA (y axis).