Figure 3.

Accumulation of open coding ends in Lig4^−/− p53^p/p thymocytes. (A) Diagram of V(D)J recombination activity at the TCRδ locus. Cleavage by Rag nucleases generates a blunt signal end (se) and hairpin coding end (ce), which can be further processed by the DNA–PK–Artemis complex. (B) LMPCR assay of WT and Lig4^−/− p53^p/p (LP) thymocytes, signal ends can be digested by ApaLI to reduce the PCR products from 123 to 98 bp. The residual species are coding ends, which was confirmed by cloning and sequencing (Fig. S6). MBN, mung bean nuclease; T4 Pol, T4 DNA polymerase. Fig. S6 is available at http://www.jem.org/cgi/content/full/jem.20062453/DC1.