Figure 2.

Foxp3⁺ cells express CD39. (A) CD4⁺ T cells from Foxp3⁺ (GFP⁺) knock-in mice stained with CD25-PE or CD39-PE. (B) Real-time PCR analysis of Foxp3⁺/CD39⁺ (black bars), Foxp3⁻/CD39⁺ (shaded bars), and Foxp3⁻/CD39⁻ (white bars) cells. (C) Foxp3⁺/CD39⁺ suppresses T effector (Foxp3⁻/CD39⁻ CD4⁺) proliferation. [³H]Thymidine incorporation was measured after 72 h. Data are representative of two independent experiments, with error bars representing the SD of triplicate wells. (D) Foxp3⁺(GFP⁺) cells stained for CD39-APC and CD73-PE. (E) CD73 mRNA expression in Foxp3⁺/CD39⁺ (black bars), Foxp3⁻/CD39⁺ (shaded bars), Foxp3⁻/CD39⁻ (white bars), and B lymphocytes (striped bars).