Figure 1.

Hyperresponsiveness of Cyld^−/− T cells. (A–C) Wild-type (+/+) and Cyld^−/− mesenteric lymph node T cells were either not treated (NT) or were stimulated with the indicated amounts of plate-bound anti-CD3 plus soluble anti-CD28. Cell proliferation (A) and cytokine production (B and C) were measured by thymidine incorporation and ELISA, respectively. Data are presented as means ± SD (error bars) of three independent experiments. (D and E) Purified naive and memory T cells were stimulated with plate-bound anti-CD3 (2.5 μg/ml for naive and 1 μg/ml for memory T cells) and anti-CD28 (2.5 μg/ml for naive and 1 μg/ml for memory T cells) for 48 h followed by measuring cell proliferation and IFN-γ production as in A and B.