Figure 3.

Induction of autoimmunity and colitis by adoptively transferred Cyld^−/− T cells. RAG1^−/− mice (8 wks old) were intravenously injected with T cells isolated from the mesenteric lymph nodes of wild-type (+/+) or Cyld^−/− mice. (A–C) After 6 wk, the recipient mice were killed for flow cytometry analyses of transferred T cells in the spleen (A), histology analyses of lymphocyte infiltration in the liver (B), and inflammation of the colon (C). The numbers in A indicate the percentages of CD8 (top left quadrants) and CD4 (bottom right quadrants) T cells. An arrow in C (bottom) indicates a colonic patch detected in a recipient of Cyld^−/− T cells. Data are representative of three mice per group. (D) Histology scores measuring the degree of colonic inflammation in the recipient wild-type (+/+) and Cyld^−/− T cells. Data were obtained from three mice per group. (E) Body weights of recipient mice. Body weights were measured 6 wk after T cell transfer, showing the substantial weight loss in the recipients of Cyld^−/− T cells. (F) Image to compare the colons of recipients of wild-type (+/+) and Cyld^−/− T cells. Error bars represent SD. Bars (B), 200 μm; (C) 1 mm.