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Western blot analysis of VCAM-1 after LPS stimulation. Lung lysates (100 μg of protein/lane) were subjected to Western blot analysis using polyclonal anti–mouse VCAM-1 antibody. GAPDH antibody was used as a loading control. Western blot analysis demonstrates an increase in VCAM-1 protein expression induced by LPS. However, the increased levels did not differ between NKCC1+/+ and NKCC1−/− mice.
