Table I.
Expression of cytokines and chemokines secreted by DCs by Luminex analysis
| Iso DC | RIIB DC | Cyt DC | RIIB DC versus Iso DC (p-value) |
RIIB DC versus Cyt DC (p-value) |
|
|---|---|---|---|---|---|
| IL-1α | 57.19 (29.4) | 699.5 (184.4) | 87.6 (80.8) | <0.001 | <0.001 |
| IL-1β | 11 (15.6) | 668.9 (780) | NE | 0.071 | NE |
| IL-2 | 10.2 (4.1) | 14.3 (4.7) | 21.9 (14) | 0.120 | 0.170 |
| IL-3 | 102.2 (16.2) | 176 (56.5) | 137.6 (35.5) | 0.023 | 0.147 |
| IL-5 | 0 (0) | 0.0 | 0 (0) | 0.000 | 0.000 |
| IL-6 | 229.6 (142.6) | 7941.8 (4116.4) | NE | 0.005 | NE |
| IL-7 | 0.1 (0.2) | 27 (3.8) | 16.4 (12.7) | 0.000 | 0.080 |
| IL-8 | 628.1 (408.1) | 10000 (0) | 5727 (4992.1) | <0.001 | 0.069 |
| IL-10 | 22.5 (2.8) | 1200.9 (987.5) | 108.6 (111.5) | 0.027 | 0.035 |
| IL-12p40 | 40.5 (28.4) | 4713.3 (5019.4) | 3191 (4483.3) | 0.056 | 0.333 |
| IL-12p70 | 15.9 (16.6) | 148.2 (148.5) | 29 (20.1) | 0.064 | 0.082 |
| IL-13 | 8.9 (3.4) | 28.9 (21.1) | 39.7 (47.2) | 0.055 | 0.345 |
| IL-15 | 3.8 (7.7) | 0.9 (1.8) | 0 (0) | 0.242 | 0.178 |
| IFN-γ | 116.5 (8.8) | 264.2 (61.1) | 198.3 (81.6) | 0.002 | 0.122 |
| TNF-α | 5.3 (6.3) | 1059.8 (911.3) | NE | 0.030 | NE |
| Eotaxin | 15.5 (19.2) | 35 (11) | 43.1 (14.5) | 0.065 | 0.204 |
| MCP1 | 1173.1 (1074) | 2776.34 (2655.6) | 1314.8 (1908.4) | 0.153 | 0.203 |
| Rantes | 27.8 (16.5) | 1531 (682) | 264.8 (356.3) | 0.002 | 0.008 |
| MIP1a | 710.6 (351.3) | 9746.9 (388) | 3067.8 (2733.7) | <0.001 | 0.001 |
| IP10 | 395.3 (185.4) | 8629.8 (2364.7) | 637.9 (664.9) | <0.001 | <0.001 |
| IFN-α | 10.2 (9.9) | 10.3 (12.5) | 28.9 (15.6) | 0.493 | 0.056 |
IDCs (n = 4) were treated with anti-FcγRIIB antibody (RIIB DC), inflammatory cytokine cocktail (Cyt DC), or isotype control antibody (Iso DC). 24 h later, the culture supernatant was collected and examined for cytokines and chemokines by Luminex assay. NE, not evaluable, as these cytokines were added exogenously to Cyt DCs.