Figure 8.

Type I IFN production in PPs by DCs after infection with reovirus. PPs from naive (open bars) or reovirus-infected BALB/c mice (2 d after infection; striped bars) were resected and fractionated to yield epithelial cells, total PP cells, MACS-enriched CD11c⁺ cells, and cells depleted of CD11c⁺ (A) or epithelial cells (Epi), total PP cells, MACS-enriched CD11c⁺ cells, cells depleted of CD11c⁺, FACS-sorted cDCs (CD11c⁺, B220⁻), and FACS-sorted pDCs (CD11c⁺ B220⁺) (B). Total RNA was purified, and IFN-α, IFN-β, and Mx-1 mRNAs were quantified using RT-qPCR. Levels of target mRNAs were normalized to GAPDH mRNA as an endogenous control. Bars are the mean of two to three replicates, and dots represent each replicate. Results are representative of at least two independent experiments.