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. 2007 Jun 11;204(6):1417–1429. doi: 10.1084/jem.20061929

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Generation of C3GnT^−/− mice. (A) The scheme shows the two major O-glycan branching pathways. C3GnT refers to C3GnT. Arrowheads show the possible pathways for further branching, elongation, fucosylation, sialylation, and sulfation. (B and C) Strategy to generate mice lacking core 3–derived O-glycans by targeting the C3GnT gene, and Southern blot genotyping using EcoRV restriction enzyme digestion. (D) RT-PCR confirmed the deletion of the C3GnT gene product. GAPDH was used as an amplification control. (E) C3GnT enzymatic activity in C3GnT ^+/+ and C3GnT ^−/− tissues. Error bars represent the mean ± the SD. n = 3. (F) LacZ staining of C3GnT ^+/+ and C3GnT ^−/− colonic tissues. Bar, 100 μm.