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. 2007 Jun 11;204(6):1417–1429. doi: 10.1084/jem.20061929

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Copyright © 2007, The Rockefeller University Press

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Figure 2. — Disruption of the C3GnT gene eliminates core 3–derived O-glycans and exposes the Tn antigen in murine colons. (A) Annotated spectra of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analyses of 2-AB–labeled O-glycans of C3GnT ^+/+ and C3GnT ^−/− colons in a linear-positive mode. Each annotated molecular mass of O-glycan includes masses of a 2-AB and a Na. Asterisks represent positions of core 3–derived O-glycans that are missing in C3GnT ^−/− mice. Data are representatives of three experiments. (B) Immunohistochemical staining of mouse colon tissue sections with a mAb to the Tn antigen. The sections were pretreated with or without sialidase. Brown reaction products mark sites of antibody binding. Data are representatives of at least three experiments. (C) C3GnT ^+/+ and C3GnT ^−/− colonic tissues stained with PAS (pink color) and Alcian blue (blue color). (D) Pixels of PAS and Alcian blue staining areas were quantified by Photoshop software based on six sections of three independent mice from each group. Error bars represent the mean ± the SD. Bars: (B) 50 μm; (C) 100 μm.