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. 2007 Jun 11;204(6):1267–1272. doi: 10.1084/jem.20062497

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Haemopoietic cells regulate mTEC development. EpCAM1⁺Ly51⁻ mTECs in 7 d FTOCs (A) can be subdivided into CD80⁻ and CD80⁺ subsets. qPCR anaysis shows mRNA for Aire is abundant in CD80⁺ mTECs (black bar) compared with CD80⁻ mTECs (white bar). The left graph in B shows percentages of CD80⁻ mTECs (▪) and CD80⁺ (▴) mTEC subsets within the total mTEC population, calculated after flow cytometric analysis of digested thymuses of the indicated ages. H-2^b CD80⁻ mTECs, shown by FACS (B) to lack surface CD80 expression and by PCR to lack CD80 mRNA (black bars, CD80⁺ mTEC; white bars, CD80⁻ mTECs), were used to make RTOCs with H-2^d thymus suspensions. RTOCs were analyzed for I-A^b (C, left), Ly51, EpCAM1, and CD80 expression after 2 d. Gating on I-A^b+ mTECs (C, right) shows CD80⁻ mTECs have generated CD80⁺ mTECs. Analysis of mTECs in FTOCs or dGuo-treated FTOCs (D) shows absence of the CD80⁺ mTEC subset in dGuo FTOCs. qPCR analysis (E) shows Aire, SP1, and SP2 expression in mTECs from FTOCs (black bars) but not dGuo-treated FTOCs (white bars).