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. 2007 Jun 11;204(6):1267–1272. doi: 10.1084/jem.20062497

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Lack of Aire⁺CD80⁺ mTECs in RANK^−/− mice promotes autoimmunity. Stroma-enriched adult thymus digests from WT (A) and RANK^−/− mice (B) were stained for CD45, EpCAM1, Ly51, and CD80. Data are gated on CD45⁻Ly51⁻ cells. In C and D, tissue sections of WT and RANK^−/− adult thymus were stained for keratin 5 (green) and Aire (red). Higher power magnification is shown in the bottom panels. Bars: (C and D, top) 20 μm; (C and D, bottom) 5 μm. E shows quantitation of Aire⁺ mTECs in sections of RANK^+/− and RANK^−/− thymus. F is qPCR highlighting absence of TRA expression in RANK^−/− thymus (white bars) compared with WT thymus (black bars). Histological analysis of leukocytic infiltrates in the liver of nude mice (arrow) transplanted with RANK^−/− dGuo-treated, but not WT thymus, is in G. (H) Immunohistochemical analysis of Rag^−/− tissues using serum obtained from RANK^−/− and WT transplanted nude mice. Red, autoantibody staining; blue, DAPI staining. Bars: G, 100 μm; H, 20 μm.