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. 2007 Jul 9;204(7):1533–1541. doi: 10.1084/jem.20062120

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Expression of CD25, CD45RO, IL-7Rα, and FOXP3 on blood CD4 T cells of healthy subjects and stable transplant recipients. Blood mononuclear cells from 45 healthy subjects and 32 stable organ transplant recipients (11 kidney and 21 liver transplant recipients) were analyzed for the surface expression of CD4, CD25, CD45RO, and IL-7Rα and for the intracellular expression of FOXP3. (A) Surface expression of CD45RO and IL-7Rα in CD4⁺CD25⁺ T cells. Representative flow cytometry profiles of one healthy donor (1 out of 45; top) and one stable liver transplant recipient (1 out of 32; bottom). The vast majority of CD4⁺CD25⁺ T cells are CD45RO⁺IL-7Rα^low and FOXP3⁺ in the healthy donor, while a substantial percentage of CD4⁺CD25⁺ T cells are CD45RO⁺IL-7Rα^high and FOXP3⁻ (green) in the stable transplant recipient. (B) Cumulative data on the proportion of CD4⁺CD25⁺ T cells (top left), CD4⁺CD25⁺FOXP3⁺ IL-7Rα^low T cells (top right), and CD4⁺CD25⁺CD45RO⁺IL-7Rα^high T cells (bottom) in stable transplant recipients and healthy donors. (C) Correlation between the expression of FOXP3 and IL-7Rα within CD4⁺CD25⁺ T cells. The percentage of FOXP3⁺ cells correlated with that of IL-7Rα^low cells within CD4⁺CD25⁺ T cells, and the percentage of FOXP3⁻ cells correlated with that of IL-7Rα^high cells within CD4⁺CD25⁺ T cells. The analyses were performed in 26 stable transplant recipients. In both cases, these correlations were statistically significant (P < 0.05). (D) Suppressive activity of IL-7Rα^high and IL-7Rα^low CD4⁺CD25⁺CD45RO⁺ T cell populations in stable transplant recipients. CD4⁺CD25⁺CD45RO⁺IL-7Rα^high and CD4⁺CD25⁺CD45RO⁺IL-7Rα^low cell populations were sorted from blood mononuclear cells of five stable liver transplant recipients, and their suppressive activity was evaluated in a MLR. The extent of cell proliferation in a MLR was assessed in the absence (positive control) or in presence of the sorted IL-7Rα^low and IL-7Rα^high cell populations. Cell proliferation was measured by [³H]thymidine incorporation. The data shown are expressed as the mean ± SE and were obtained from five independent experiments.