Figure 3.

Analysis of allospecific CD4 T cell responses. Sorted purified CD4⁺CD25⁺CD45RO⁺IL-7Rα^high T cells (7 × 10⁴ cells) from two time points of a kidney transplant recipient with biopsy-proven chronic rejection were co-cultured with 10⁵ irradiated blood mononuclear cells (40 Gy) either from the organ donor or from an MHC unrelated subject. After 7 d of culture, cell proliferation was measured by [³H]thymidine incorporation. Cell cultures containing only irradiated cells were used as negative controls.