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. 2007 Jul 9;204(7):1653–1664. doi: 10.1084/jem.20062648

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — The loss of splenic CD8⁻ DCs in mice with DC-specific RBP-J deletion. Spleens from control (RBP-J^fl/fl) and CKO (RBP-J^fl/fl CD11c-Cre⁺) mice were analyzed for the indicated cell populations. (A) Staining profiles of PDCs and conventional DCs, with the percentages among total splenocytes indicated (mean ± SD of 3 and 17 animals per genotype, respectively). Within the DC population, the mean percentages of CD8⁻ and CD8⁺ DC subsets and the CD8⁻/CD8⁺ DC ratio are shown. (B) Absolute numbers of CD8⁻ and CD8⁺ DCs in individual control (squares) and CKO (triangles) mice. Horizontal lines represent the means. (C) Staining of splenic DCs with the CD8⁻ DC–specific mAb 33D1, with the fractions of 33D1⁺ and 33D1⁻ DCs among total splenocytes indicated. A threefold decrease in the absolute number of 33D1⁺ DCs was observed in CKO spleens (not depicted). (D and E) DC populations in the cutaneous lymph nodes (D) and the thymus (E). Shown are the staining profiles of lineage-depleted cells, with blood-derived (CD11c^high MHC class II⁺) and mature tissue-derived (CD11c⁺ MHC class II^high) DC populations highlighted. The percentages of these populations among the total cells of each organ are indicated (mean ± SD of four mice). Within the blood-derived DC population, the fractions of CD8⁻ and CD8⁺ subsets are shown (mean ± SD of four mice). (F) Staining profiles of alveolar macrophages (CD11c^high MHC class II⁺) and conventional resident DCs (CD11c^low MHC class II^high) in the lung. The percentage of each population among total lung cells is indicated (mean ± SD of four mice).