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. 2007 Jul 9;204(7):1653–1664. doi: 10.1084/jem.20062648

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Localization of CD8⁻ DCs and Dll1-expressing cells in the spleen. (top) Spleen sections from wild-type control (RBP-J^fl/fl) or CKO (RBP-J^fl/fl CD11c-Cre⁺) mice were stained for metallophilic macrophages delineating the MZ (MOMA-1⁺; green) and CD8⁻ DCs (33D1; red). A serial section of control spleen was stained for MOMA1 (green) and Dll1 (red). (middle and bottom) Wild-type spleen sections were stained for CD8⁻ DCs (33D1; green) and Dll1 (red). The location of 33D1⁺ cells in the MZ was confirmed by MOMA-1 staining of a serial section. Shown are the representative individual and merged images of 33D1 and Dll1 staining (middle) and a high magnification image of the area indicated by the dashed box (bottom). Bars: (top) 400 μm; (middle) 200 μm; (bottom) 100 μm.