Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jul 9;204(7):1613–1623. doi: 10.1084/jem.20062525

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Hypertrophic changes are associated with elevated ROS. (A, top) ATDC5 cells cultured in control medium (a), differentiation medium (b), or differentiation medium plus NAC (c) for 3 wk were stained using rabbit anti–type X collagen antibody, followed by Alexa Fluor 488–conjugated anti–rabbit Ig antibody, and observed under a fluorescence microscope. (bottom) Data are mean numbers ± SD of type X collagen–positive hypertrophic chondrocyte–containing nodules. Bar, 50 μm. (B) ATDC5 cells were cultured in differentiation medium, control medium, or differentiation medium plus antioxidant NAC for 1–4 wk, and the expression of mmp13, type X collagen, runx2, and β-actin was determined by RT-PCR.