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. 2007 Jul 9;204(7):1553–1558. doi: 10.1084/jem.20070823

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Purkinje cell death in Pcp2-Cre; Dicer^floxflox mice. Electron microscopy images of degenerating Dicer-deficient Purkinje cells (A–E). Degenerating cells display condensation of the cytoplasm in cell bodies (A) and the dendritic compartment (B). Cytoplasmic condensation is associated with the occurrence of protein accumulations (A), autophagic vacuoles, and membrane whorls (B). Region of interests in A are marked by squares and shown in more detail (C–E). Membranes surrounding protein accumulations (C and E) and intracisternal inclusions (C–E) are indicated by arrows. The 12-μm-thick cerebellar section of a 13-wk-old Pcp2-Cre; Dicer^flox/flox mouse is shown in F–H. Purkinje cells were visualized using anti-calbindin antibody (red), and apoptotic cells were detected by TUNEL staining (green). A TUNEL⁺ degenerating Purkinje cell (indicated by a dashed line) can be seen close to a TUNEL⁻ normal-appearing Purkinje cell (arrow). Bars: A, 5 μm; B, 0.2 μm; C–E, 1 μm; F–H, 10 μm.