Figure 2.

Ligation of Fcγ receptors on the priming DCs augments Th2-mediated airway inflammation and cytokine production in vivo. BMDCs generated from B6 mice were cultured on a nonspecific control rat IgG (open bars) or anti-FcγRIIb/RIII (2.4G2) (filled bars) –coated plates along with OVA. After 24 h, these DCs were harvested and instilled i.t. into naive B6 recipient mice. After sensitization, the recipient mice were challenged with soluble OVA i.t. daily for 3 d starting on day 7, and BAL was performed 24 h after last challenge. (A) Airway inflammation was assessed by determining the cellular composition of the BAL fluid (*, P < 0.05; **, P < 0.01; ***, P < 0.001). The error bars indicate variation between individual mice. (B) For each group, the BAL cells were pooled and cytokine production of the CD4⁺ T cells was determined by intracellular staining. (C) Pooled lung cells were restimulated in vitro with OVA for 48 h, and cytokine production was assessed by ELISPOT analysis (**, P < 0.01; ***, P < 0.001). The error bars indicate the SEM between replicate stimulations in different wells. The data represented in this figure were obtained from three independent experiments, and 9–10 mice per group were analyzed.