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. 2007 Aug 6;204(8):1741–1748. doi: 10.1084/jem.20070193

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Physical interaction between CypA and AIF after HI in vivo and in vitro. (A) Coimmunoprecipitation of CypA and AIF in ischemic but not in nonischemic brain tissue. Immunoprecipitation of AIF in nonischemic control and ipsilateral, HI cortex homogenates 3 h after HI. (B) Surface plasmon resonance measurement of the CypA–AIF interaction. The curve demonstrates the net resonance signal in RU obtained by subtracting the reference channel from the experimental channel. Recombinant AIF protein was captured by an immobilized antibody raised against AIF, resulting in a 320-RU rise. Subsequent injection of CypA resulted in a 123-RU rise, indicating CypA binding to AIF. This binding was reversible, because CypA dissociated upon change to buffer flow until the initial plateau value was reached. The dissociation constant was calculated to ∼1 μM. Repeated injections of CypA resulted in the same association and dissociation phases as found in the first injection.