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. 2007 Aug 6;204(8):1989–1998. doi: 10.1084/jem.20070902

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Homozygous PCNA^K164R mice are born at sub-Mendelian frequency and are infertile. (A) 397 offspring from 70 intercrosses between heterozygous PCNA^K164R mutants were genotyped. In contrast to the 25% expected homozygous mutants, only 5% were observed. Wild-type and heterozygous mice were born at a frequency of 34 and 61%, respectively. (B) Embryos from E14.5 intercrosses between heterozygous PCNA^K164R mutants with the respective Applied Biosystems sequencing profile of their PCNA alleles are shown. (C) Failure of germ cell development in homozygous PCNA^K164R mice. Histological sections of testis and ovary of 3-mo-old mice. Bars: (small) 500 μm; (large) 200 μm. (top four panels) Control or PCNA^K164R testes. The control testis shows all stages of normal spermatogenesis whereas PCNA^K164R testes show atrophy of spermatogenesis: only Sertoli cells are found, no sperm is detected. The PCNA^K164R testes show strong hyperplasia of Leydig cells. (bottom four panels) The normal ovary contains numerous follicles in all stages of development. The PCNA^K164R ovary consists predominantly of interstitial cells.