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. 2007 Aug 6;204(8):1825–1835. doi: 10.1084/jem.20070872

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Restoration of FBW7 expression in CEM cells (FBW7^MUT) suppresses NOTCH1 target genes. (A and B) Efficient restoration of FBW7^WT in CEM T-ALL cells after transduction with a Flag-FBW7α–expressing retrovirus (MigR1-FBW7α). Cells were infected with empty MigR1 vector as a control. (A) RT-PCR amplified Flag-FBW7α only in cells infected with MigR1-FBW7α. (B) Immunofluorescence staining of FACS-sorted CEM cells infected with MigR1-FBW7α using FITC-conjugated anti-Flag antibody shows nuclear localization of the construct. (C) Real-time RT-PCR was used to quantify the expression of the NOTCH1 target genes, DELTEX1, and HES1 in CEM cells infected as in A Infection with MigR1-FBW7α suppressed the two genes (P < 0.0001 for both). Error bars represent the SD of triplicate wells.