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. 2007 Aug 6;204(8):1945–1957. doi: 10.1084/jem.20070133

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — CD8SP cells use the distal promoter of Runx3. (A) Runx3 protein is predominantly expressed in CD8SP thymocytes in the thymic medulla. Frozen sections from a wild-type thymus were stained for CD4 (blue), CD8 (red), and Runx3 (green). Arrowheads indicate cells expressing both CD8 and Runx3. (B) Genomic organization of exons encoding the different mouse Runx3 isoforms. White boxes and filled boxes depict coding and noncoding sequences, respectively. (C) Western blot analysis of total Runx3 expression in CD4⁺ and CD8⁺ T cells from lymph nodes. Immunoblot against HMG1 was used as a loading control. (D) Western blot analysis to detect the distal promoter-derived Runx3 protein in CD8⁺ T cells. (E) RT-PCR analysis of promoter-specific Runx3 mRNA expression in different subsets of thymocytes and T cells. Numbers shown below each lane are the relative amount of Actb cDNA determined by real-time PCR.