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. 2007 Sep 3;204(9):2131–2144. doi: 10.1084/jem.20070070

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Transcription of CLIC-2 in blood cell subsets. (A) Total RNA was extracted from the PBMCs of 16 healthy donors and 16 SoJIA patients; B cells, T cells, and monocytes from 3 healthy donors and 4 SoJIA patients, and neutrophils from 6 healthy donors and 7 SoJIA patients. Amplified cRNA was hybridized on Affymetrix HG-U133 chips. Raw intensity values from each chip were first pre-scaled to the 500 target intensity value in Affymetrix Microarray suite before being imported and analyzed in GeneSpring 6.1. (B) Total RNA was extracted from the PBMCs of 16 healthy donors and 16 SoJIA patients, mDCs from 9 healthy donors, and pDCs from 6 healthy donors. Amplified cRNA was hybridized to Affymetrix HG-U133 chips. Raw intensity values from each chip were pre-scaled to the 500 target intensity value in Affymetrix Microarray suite before being imported and analyzed in GeneSpring 6.1.