Figure 2.

Splenocytes from MOG-immunized male PPARα^−/− mice secreted higher levels of IFN-γ and TNF compared with WT counterparts. Splenocytes from male and female SV.129 WT or PPARα^−/− mice were harvested 10 d after EAE induction and stimulated with 0–20 μg/ml MOG p35-55. (A) Proliferation of cells in response to 5 μg/ml MOG peptide was measured by [³H]thymidine incorporation (cpm). Values are means ± SEM counts of radioactivity per minute (cpm) of triplicate culture wells. (B) Cytokine levels in culture supernatants were measured by ELISA at 48 (IL-17), 72 (IFN-γ and TNF), and 120 h (IL-10) after stimulation. Values are means ± SEM of cytokine levels (pg/ml) in triplicate culture wells. Note that IL-4 was not detected in culture supernatants. Results are representative of two to three independent experiments.