Table I.
Clinical and histological features of EAE in male and female WT and PPARα−/− mice
| WT male |
PPARα−/−
male |
WT female |
PPARα−/−
female |
|
|---|---|---|---|---|
| Clinical features | ||||
| Maximum score | ||||
| Experiment 1 | 2.5 (0.3) | 3.8 (0.4)a | 1.9 (0.2) | 1.9 (0.2) |
| Experiment 2 | 2.4 (0.5) | 3.5 (0.3)a | 2.7 (0.6) | 2.9 (0.3) |
| Death from disease | ||||
| Experiment 1 | 0/8 | 3/7 | 0/18 | 0/15 |
| Experiment 2 | 1/8 | 1/8 | 2/9 | 1/9 |
| Incidence | ||||
| Experiment 1 | 8/8 | 7/7 | 17/18 | 14/15 |
| Experiment 2 | 8/8 | 8/8 | 7/9 | 9/9 |
| Day of onset | ||||
| Experiment 1 | 14.2 (1.2) | 12.8 (0.3) | 13.9 (0.8) | 14.5 (1.0) |
| Experiment 2 | 16.4 (1.7) | 12.2 (0.2)a | 14.4 (1.8) | 11.1 (0.5) |
| Cumulative scoreb | ||||
| Experiment 1 | 12.5 (2.3) | 25.3 (5.2)a | 9.2 (1.4) | 10.3 (1.6) |
| Experiment 2 | 16.6 (6.2) | 23.3 (9.5) | 19.4 (7.0) | 22.8 (6.1) |
| Histological features (Experiment 2) | ||||
| Parenchymal inflammatory foci | ||||
| Cerebellum | 15 (8) | 31 (13) | 12 (2) | 12 (7) |
| Brain stem | 10 (4) | 23 (1)a | 22 (11) | 8 (4) |
| Cerebrum | 16 (6) | 39 (14) | 14 (4) | 2 (1)a |
| Spinal cord | 23 (15) | 49 (6) | 23 (9) | 32 (18) |
| Total | 64 (33) | 142 (27) | 71 (21) | 54 (31) |
| Meningeal inflammatory foci | ||||
| 139 (55) | 147 (7) | 123 (11) | 58 (12)a | |
Values are means (SEM).
a
A significant difference from WT counterpart; P < 0.05.
b
Up until day 22 (approximate time of remission after acute phase).