Table I.

EC₅₀ values for human and mouse chimeric TLR5 molecules stimulated with wild-type and mutant flagellins

^aSchematic of chimeric TLR5 molecules with amino acid numbers corresponding to mouse TLR5.

^bEC₅₀ values and standard deviations are listed in ng/ml, and values were calculated from at least three independent experiments, each run in duplicate.

^cEC₅₀ values for each flagellin point mutant were compared to wild-type flagellin, and p-values were calculated using a two-tailed Student's t test. p-values >0.05 are not shown.

^dThe pattern of response for each TLR5 chimera to the mutant flagellins was compared to parental human and mouse TLR5, and the overall flagellin recognition phenotype is listed.