Figure 3.

Shc activation in response to LPA in L cells. Confluent L cells were serum-starved in phosphate-deficient medium for 16 h, pulsed with 500 μCi/ml ³²P-orthophosphate for 3 h, and stimulated with 1% BSA (15 min, CO), 25 μM LPA (indicated time points), or 10 ng/ml PDGF-BB (15 min). Incorporation of ³²P into Shc was measured by autoradiography and densitometric scanning. Shc isoforms of different molecular masses are indicated (p66, p52). The filter was reprobed with anti-Shc (αShc).