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Effect of AG1296 and AG1478 on LPA-induced ERK activation in L cells. Confluent, serum-starved L cells were pretreated with DMSO, AG1296, or AG1478 at the indicated concentrations. Before lysis, the cells were incubated for 5 min with 1% BSA (control, CO), 25 μM LPA, or 10 ng/ml PDGF-BB. ERK activation was detected with an ERK mobility shift assay (see Materials and Methods).
