Figure 3.

Time course of renin release from microdissected glomeruli-AA. The glomeruli-AA were incubated in Medium 199 with 0.1% BSA for 0.5, 1, and 2 h (control, black bars) or for the same times in medium containing 10 μM forskolin (grey bars). Renin release data are expressed as percentage of renin release, i.e., the ratio of released renin/total renin (total sum of released renin plus that remaining in the cells) as described (17). Data represent the mean ± SEM from three different experiments. Forskolin samples were significantly different (∗, P < 0.01) from their respective controls at each time point. From these data, 1 h was chosen as the incubation time for all other experiments with glomeruli-AA.