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. 1998 Jul 21;95(15):9003–9008. doi: 10.1073/pnas.95.15.9003

Figure 4.

Figure 4

Inhibition of forskolin-stimulated renin release from microdissected glomeruli-AA by a selective activator of cGK. Glomeruli were incubated 1 h in Medium 199 with 0.1% BSA either in the absence or presence of 10 μM forskolin for 1 h as shown. Preincubation of some samples for 5 min with the cGK-selective activator 8-pCPT-cGMP (2.5 μM) before forskolin was added caused a major inhibition of forskolin-stimulated renin release (lane 4). In other samples, a cGK-selective inhibitor, Rp-8-pCPT-cGMPS (25μM) was added 15 min before the addition of 8-pCPT-cGMP (lanes 5 and 6) or forskolin (lane 7). To minimize variation among different glomeruli preparations, renin secretion was expressed as percentage of renin release, as described in Fig. 3. Data represent the mean ± SEM from three different experiments. Designated samples (∗) are significantly different (P < 0.01) from untreated controls (lane 1), cGMP analogs which alone had no significant effect on renin release in the absence of forskolin (lanes 2 and 6), and the forskolin-stimulated renin release, which was inhibited by 8-pCPT-cGMP (lane 4).