Figure 5.

Inhibition of forskolin-stimulated renin release from microdissected glomeruli-AA by NO donors. Glomeruli were incubated 1 h in Medium 199 with 0.1% BSA either in the absence or presence of 10 μM forskolin as shown. Preincubation of glomeruli with either SIN-1 (10 μM) or SNP (50 μM) 5 min before forskolin was added resulted in a partial inhibition of forskolin-stimulated renin release, which was less than that observed with 8-pCPT-cGMP. Results are presented as percentage of renin release as described in Fig. 3. Data represent the mean ± SEM from three different experiments. All designated (∗ or ∗∗) samples were significantly different (P < 0.01) from control, and forskolin-treated glomeruli (∗∗) were significantly different from those pretreated with SIN-1 or SNP (P < 0.05).