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. 1998 Jul 21;95(15):9014–9018. doi: 10.1073/pnas.95.15.9014

Figure 2.

Figure 2

HR induced after injection of purified ECP2 in a leaflet of line 1. (A) Purification of the ECP2 protein. Lane M contains molecular weight markers, lane 1 contains 50 μl of AF, and lane 2 contains the purified ECP2 protein. (B) HR induced after injection of ECP2 in the apoplast of a leaflet of a line 1 plant (Lower) and absence of HR after injection of ECP2 in the apoplast of a leaflet of the Moneymaker cultivar (Upper). Only the four lines identified by inoculation with PVX∷Ecp2 responded by chlorosis, followed by necrosis in the injected area three days after injection of ECP2; all other lines showed no detectable response after ECP2 injection.