Figure 4.

Immunodetection of ECP2 in AFs isolated from cultivar Moneymaker inoculated with 25 strains of C. fulvum obtained from a worldwide collection. A volume of AF containing 4 μg of protein was separated on denaturing polyacrylamide gel, blotted, and incubated with polyclonal antibodies raised against ECP2. ECP2 is detected in each lane. HR-inducing activity of each AF sample was assayed by injecting 5–10 μl into a leaflet of line 1. HR activity visible 3 days after injection is indicated by “+.”