Figure 4.

Complementation of the ga3-2 mutant. (A) Photographs of three kanamycin resistant plants from the transformation of ga3-2 with a genomic clone containing the P450 gene (I-III), the ga3-2 ttg line and wild-type L.er. All plants were germinated on medium containing GA₃ (the seeds from the transformation were germinated on medium that also contained kanamycin), transferred to medium without GA after cotyledons were expanded (11 days) and transferred to soil after a further 13 days growth. Photographs were taken 10 days after transfer to soil. (B) PCR analysis of kanamycin resistant plants. PCR was carried out using primers specific to the L.er and Col copies of the gene. L.er specific products were digested with EcoRI to distinguish between ga3-2 and GA3 copies of the gene.