Figure 2.

Anisomycin and sparsomycin specifically affect −1 ribosomal frameshifting efficiencies in vivo. JD88 cells containing the frameshift indicator plasmids pTI25, pF8, or pJD104 were cultured in selective medium in the presence of the indicated antibiotic concentrations for 5 hr, after which β-gal activities were determined. The filled symbol represents the average of at least three independent experiments. Open symbols represent high and low margins of error. (A and B) β-gal activities produced from pTI25 as a percentage of the no-drug controls. (C and D) The fold changes in −1 or +1 ribosomal frameshifting efficiencies (RFS) as compared with the no-drug controls [−1 (solid line) = 1.8%; +1 (dashed line) = 5.5%].