Figure 3.

Anisomycin and sparsomycin promote virus loss. JD88 cells were grown in rich medium in the presence of the indicated concentrations of drugs for 1–5 days, and aliquots of cells were removed every 24 hr, streaked for single colonies onto rich medium lacking drugs, and replicated to killer indicator plates. (A and B) Rates of killer loss were calculated as described in Materials and Methods. Anisomycin and sparsomycin concentrations (μM) are indicated. (C) Total nucleic acids were extracted from control (Killer⁺) and non-Killer colonies, separated by agarose gel electrophoresis, transferred to nitrocellulose and hybridized with L-A and M₁ (+) strand probes, and visualized by autoradiography as described in Materials and Methods.