Figure 5.

Anisomycin and sparsomycin affect −1 ribosomal frameshifting efficiencies in a rabbit reticulocyte in vitro translation system. Synthetic 7-methyl-Gppp-capped, polyadenylylated mRNAs were transcribed from DraI-digested pLUC0 and pLUC-1, and 20 ng of LUC0 or LUC-1 mRNA were used for each in vitro translation reaction in the presence of the indicated concentrations of anisomycin or sparsomycin. The open squares represent the average of at least three independent experiments. High and low margins of error are indicated. A (anisomycin) and B (sparsomycin): Changes in 0-frame luciferase as the fold of no-drug controls. C (anisomycin) and D (sparsomycin): % −1 ribosomal frameshifting.