Table 1.
Quantitative analysis of the Tau-1 epitope on immunoblots of OVX female rat cerebrum 0–12 hr after heat shock with and without daily replacement therapy with EB [20 μg/100 g (body weight)] for 2–3 weeks
|
125I-labeled protein A, cpm × 10−3/100 μg of extract protein
|
|||||
|---|---|---|---|---|---|
| Control | hr after heat shock
|
||||
| 0 | 3 | 6 | 12 | ||
| Sham-OVX + SO | n = 10 | n = 5 | n = 6 | n = 8 | n = 5 |
| Nonphosphorylated | 14.0 ± 1.7 | 29.2 ± 2.5 | 7.4 ± 1.1 | 11.3 ± 1.7 | 17.1 ± 1.2 |
| (0.0001) | (0.0020) | ||||
| Total | 43.1 ± 1.8 | 48.9 ± 5.1 | 39.6 ± 2.0 | 42.0 ± 2.7 | 44.6 ± 1.5 |
| Total/nonphosphorylated | 3.5 ± 0.4 | 1.7 ± 0.1 | 5.7 ± 0.5 | 4.4 ± 0.8 | 2.7 ± 0.3 |
| (0.0319) | |||||
| OVX + SO | n = 5 | n = 4 | n = 5 | n = 5 | n = 5 |
| Nonphosphorylated | 10.1 ± 1.3 | 16.5 ± 4.5 | 4.7 ± 1.6 | 5.0 ± 1.0 | 12.0 ± 0.8 |
| (0.0188) | (0.0370) | (0.0469) | |||
| Total | 31.8 ± 3.6 | 29.6 ± 9.5 | 25.8 ± 6.0 | 26.6 ± 5.8 | 36.6 ± 2.8 |
| Total/nonphosphorylated | 3.3 ± 0.4 | 1.7 ± 0.2 | 6.9 ± 1.5 | 6.7 ± 1.9 | 3.1 ± 0.3 |
| (0.0055) | (0.0084) | ||||
| OVX + EB | n = 5 | n = 4 | n = 6 | n = 5 | n = 3 |
| Nonphosphorylated | 7.7 ± 0.8 | 23.2 ± 0.9 | 5.0 ± 1.0 | 4.9 ± 0.8 | 13.7 ± 0.5 |
| (0.0001) | (0.0426) | ||||
| Total | 26.4 ± 1.4 | 33.8 ± 1.8 | 29.6 ± 1.7 | 32.5 ± 5.3 | 32.0 ± 2.0 |
| Total/nonphosphorylated | 3.5 ± 0.2 | 1.5 ± 0.1 | 7.1 ± 1.3 | 7.0 ± 1.0 | 2.3 ± 0.1 |
| (0.0042) | (0.0069) | ||||
Nonphosphorylated and total are defined as the epitope recognized by Tau-1 without and with alkaline phosphatase treatment of electroblotted proteins before immunoblotting, respectively. Data are mean ± SE values. Shown in parentheses are the values of P < 0.05 in comparison with the respective control. n, number of rats.