Figure 1.

One-dimensional TRNOE difference spectra of an oligodeoxyribonucleotide. (a and b) Spectra of 1.1 mM d(TGACAT) and 54 μM RecA protein in the presence of 1.1 mM ATPγS (a) or ADP (b) in D₂O at 37°C. (c) Spectrum of the DNA solution before the addition of RecA protein and ATPγS or ADP. (d) DNA was replaced by RNA; 1.1 mM r(UGACAU) in the presence of RecA protein and ATPγS. The cytosine H5 proton of DNA or RNA was irradiated for 0.5 sec before a 90° read pulse.