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. 1997 Jun 24;94(13):6635–6640. doi: 10.1073/pnas.94.13.6635

Figure 2.

Figure 2

Optical spectrum of apo Trx-Fe4S4 (trace A), holo Trx-Fe4S4 (trace B) in 15 mM Mops/100 mM NaCl (pH 7.4) at 5°C, and the synthetic cluster [Fe4S4(S-EtOH)4](Me4N)2 (trace C) in 10 mM Ches/10 mM βME (pH 8.5). holo Trx-Fe4S4 contains 3.9 ± 0.2 mol of iron and 3.9 ± 0.2 mol acid labile sulfide per Trx protein; λmax = 280 nm, ɛM,280 = 36,000 λmax = 413 nm, ɛM,413 = 16,100. (Inset) CD spectra of apo Trx-Fe4S4 (upper trace) and Trx (lower trace) in 50 mM potassium phosphate (pH 7.0) at 25°C. The upper trace has been offset slightly to clarify the close superposition of the two curves.