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Stability of the free synthetic cluster [Fe4S4(S-EtOH)4](Me4N)2 (□) in the presence of low levels (0.4 mM) of stabilizing exogenous βME and reconstituted holo Trx-Fe4S4 (▪) in the absence of stabilizing exogenous βME. Solution conditions: 10 mM Mops/100 mM NaCl (pH 7.4) at 5°C.
