Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1987 May;169(5):2132–2136. doi: 10.1128/jb.169.5.2132-2136.1987

Regulation of dipeptide transport in Saccharomyces cerevisiae by micromolar amino acid concentrations.

M D Island, F Naider, J M Becker
PMCID: PMC212111  PMID: 3553158

Abstract

Prototrophic Saccharomyces cerevisiae X2180, when grown on unsupplemented minimal medium, displayed little sensitivity to ethionine- and m-fluorophenylalanine-containing toxic dipeptides. We examined the influence of the 20 naturally occurring amino acids on sensitivity to toxic dipeptides. A number of these amino acids, at concentrations as low as 1 microM (leucine and tryptophan), produced large increases in sensitivity to leucyl-ethionine, alanyl-ethionine, and leucyl-m-fluorophenylalanine. Sensitivity to ethionine and m-fluorophenylalanine remained high under either set of conditions. The addition of 0.15 mM tryptophan to a growing culture resulted in the induction of dipeptide transport, as indicated by a 25-fold increase in the initial rate of L-leucyl-L-[3H]leucine accumulation. This increase, which was prevented by the addition of cycloheximide, began within 30 min and peaked approximately 240 min after a shift to medium containing tryptophan. Comparable increases in peptidase activity were not apparent in crude cell extracts from tryptophan-induced cultures. We concluded that S. cerevisiae possesses a specific mechanism for the induction of dipeptide transport that can respond to very low concentrations of amino acids.

Full text

PDF
2132

Images in this article

2134Image
on p.2134

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews J. C., Blevins T. C., Short S. A. Regulation of peptide transport in Escherichia coli: induction of the trp-linked operon encoding the oligopeptide permease. J Bacteriol. 1986 Feb;165(2):428–433. doi: 10.1128/jb.165.2.428-433.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andrews J. C., Short S. A. opp-lac Operon fusions and transcriptional regulation of the Escherichia coli trp-linked oligopeptide permease. J Bacteriol. 1986 Feb;165(2):434–442. doi: 10.1128/jb.165.2.434-442.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bekcer J. M., Naider F. Peptide transport in yeast: uptake of radioactive trimethionine in Saccharomyces cerevisiae. Arch Biochem Biophys. 1977 Jan 15;178(1):245–255. doi: 10.1016/0003-9861(77)90189-8. [DOI] [PubMed] [Google Scholar]
  4. Davies M. B. Peptide uptake in Candida albicans. J Gen Microbiol. 1980 Nov;121(1):181–186. doi: 10.1099/00221287-121-1-181. [DOI] [PubMed] [Google Scholar]
  5. Fujita M., Parsons D. S., Wojnarowska F. Oligopeptidases of brush border membranes of rat small intestinal mucosal cells. J Physiol. 1972 Dec;227(2):377–394. doi: 10.1113/jphysiol.1972.sp010038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jamieson D. J., Higgins C. F. Anaerobic and leucine-dependent expression of a peptide transport gene in Salmonella typhimurium. J Bacteriol. 1984 Oct;160(1):131–136. doi: 10.1128/jb.160.1.131-136.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Logan D. A., Becker J. M., Naider F. Peptide transport in Candida albicans. J Gen Microbiol. 1979 Sep;114(1):179–186. doi: 10.1099/00221287-114-1-179. [DOI] [PubMed] [Google Scholar]
  8. Marder R., Rose B., Becker J. M., Naider F. Isolation of a peptide transport-deficient mutant of yeast. J Bacteriol. 1978 Dec;136(3):1174–1177. doi: 10.1128/jb.136.3.1174-1177.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCarthy P. J., Nisbet L. J., Boehm J. C., Kingsbury W. D. Multiplicity of peptide permeases in Candida albicans: evidence from novel chromophoric peptides. J Bacteriol. 1985 Jun;162(3):1024–1029. doi: 10.1128/jb.162.3.1024-1029.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moneton P., Sarthou P., Le Goffic F. Transport and hydrolysis of peptides in Saccharomyces cerevisiae. J Gen Microbiol. 1986 Aug;132(8):2147–2153. doi: 10.1099/00221287-132-8-2147. [DOI] [PubMed] [Google Scholar]
  11. Naider F., Becker J. M., Katzir-Katchalski E. Utilization of methionine-containing peptides and their derivatives by a methionine-requiring auxotroph of Saccharomyces cerevisiae. J Biol Chem. 1974 Jan 10;249(1):9–20. [PubMed] [Google Scholar]
  12. Wolfinbarger L., Jr, Marzluf G. A. Specificity and regulation of peptide transport on Neurospora crassa. Arch Biochem Biophys. 1975 Dec;171(2):637–644. doi: 10.1016/0003-9861(75)90074-0. [DOI] [PubMed] [Google Scholar]
  13. Yadan J. C., Gonneau M., Sarthou P., Le Goffic F. Sensitivity to nikkomycin Z in Candida albicans: role of peptide permeases. J Bacteriol. 1984 Dec;160(3):884–888. doi: 10.1128/jb.160.3.884-888.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES