Figure 2.

MIF inhibits cytodifferentiation and hemoglobin synthesis of MEL cells. (A) Photomicrographic images of benzidine-stained cells from three experiments (I, II, and III) cultured with or without differentiation medium for 96 h together with 200 ng/ml recombinant mouse MIF or MIF plus 100 μg/ml anti-MIF mAb. (B) Intracellular hemoglobin quantification of lysed MEL cells (4 × 10⁵ cells per experiment) as described in Materials and methods. Each value represents the mean ± SD (error bars) of five different experiments. *, P < 0.01 versus control.