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. 2006 May 15;203(5):1197–1207. doi: 10.1084/jem.20060418

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Vα14tg.CD1d^−/− DN T cells have diverse CDR3 sequences. Vβ8.1- (n = 35), Vβ8.2- (n = 58), and Vβ12-CDR3 (n = 96) sequences amplified by RT-PCR from sorted Vα14tg.CD1d ^−/− DN T cells were cloned and sequenced. (A) Bar graphs of CDR3 lengths (a.a., amino acid). (B) Frequencies of Jβ usage expressed as percentage of total sequences. Note that a similar bias in Jβ2 cluster usage has been reported for conventional T cells. (C) Number of unique and duplicate sequences among Vβ8.1, Vβ8.2, and Vβ12.